Studies on the Sugar Chains of Interferon-γ from Human Peripheral-Blood Lymphocytes1

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Abstract
Sugar chains of interferon-γ (IFN-γ) from human peripheral-blood lymphocytes (PBL) were liberated by hydrazinolysis. After N-acetylation, the reducing end residues of the sugar chains were tagged with 2-aminopyridine and the pyridylamino (PA-) derivatives were purified by gel filtration and reversed-phase HPLC. Five major PA-sugar chains were obtained. The structure of each PA-sugar chain was estimated by comparing its elution times on anion exchange, reversed-phase, and size-fractionation HPLC with those of PA-sugar chains of IFN- γ from the human myelomonocyte cell line HBL-38 (Yamamoto, S. et al. (1989) J. Biochem. 105, 547–555) as standards, and also by comparison of their elution times after partial desialylation. The results showed that IFN-γ (PBL) contained mono-and disialo-biantennary structures with 0 or 1 mol of fucose residue, as found for IFN-γ (HBL-38), but the N-acetylneuraminyl α2–6 linkage was dominant in IFN-γ (PBL), unlike IFN-γ (HBL-38), which contains both N-acetylneuraminyl α2–3 and α2–6 linkages.

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