High performance liquid chromatography determination of ergosterolas a measure of ectomycorrhiza infection in Scots pine

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Abstract
A technique for determining the ergosterol content in mycorrhizal pine roots using HPLC was developed. Pure cultures of different my corrhizal fungi contained very similar and constant amounts of ergosterol, and calculations of fungal biomass in mycorrhizal roots based on ergosterol readings agreed well with results using other methods. The extraction and sample purification were simple and reliable. Consequently, the technique is considered practical wherever accurate estimates of the intensity of mycorrhizal infection are required.