Phleomycin resistance as a dominant selectable marker in CHO cells
- 1 May 1988
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 14 (3) , 243-252
- https://doi.org/10.1007/bf01534585
Abstract
The Tn5 and the Streptoalloteichus hindustanus (Sh) ble genes conferring resistance to bleomycin-phleomycin antibiotics have been cloned into a mammalian vector under the RSV-LTR promoter. The resulting plasmids, pUT506 and pUT507 respectively, were used to transfect CHO cells by either the calcium phosphate or the recently described polybrene-DMSO method. Phleomycin- or bleomycin-resistant clones arose with a higher frequency after transfection with pUT507, and pUT507 transfectants were more resistant to both antibiotics than pUT506 transfectants. Phleomycin resistance in pUT507 transfectants was stable and associated with integration of plasmid sequences in genomic DNA. The Sh ble gene, which confers a dominant phleomycin-resistance phenotype, should provide a useful transferable selectable marker in CHO cells as well as in other animal cell lines.Keywords
This publication has 36 references indexed in Scilit:
- Expression in mammalian cells of a gene fromStreptomyces albonigerconferring puromycin resistanceNucleic Acids Research, 1986
- Construction of a fusion gene that confers resistance against hygromycin B to mammalian cells in cultureExperimental Cell Research, 1985
- Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cellsGene, 1984
- The cloning and reintroduction into animal cells of a functional CAD gene, a dominant amplifiable genetic markerCell, 1981
- A new dominant hybrid selective marker for higher eukaryotic cellsJournal of Molecular Biology, 1981
- Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.Proceedings of the National Academy of Sciences, 1981
- Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase.Proceedings of the National Academy of Sciences, 1981
- Expression of a Bacterial Gene in Mammalian CellsScience, 1980
- Transformation of mammalian cells with an amplifiable dominant-acting gene.Proceedings of the National Academy of Sciences, 1980
- Transfer of purified herpes virus thymidine kinase gene to cultured mouse cellsCell, 1977