Isolation of theMaclura pomiferahemagglutinin on a deoxymelibiotol affinity support and preliminary characerization by buffer electrofocusing and high-performance liquid chromatography
- 1 April 1982
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 2 (4) , 247-255
- https://doi.org/10.1007/bf01136724
Abstract
Maclurin, the potent nonspecific blood-group hemagglutinin present in extracts of M. pomifera, was purified by a new biospecific affinity-chromatographic procedure. This hemagglutinin apparently occurs as 5 closely related tetrameric protein isoforms derived from 2 non-covalently-linked polypeptide chains, MW .apprx. 10,000 and 13,000, respectively. Buffer electrofocusing fractionated the lectin into 12 components; the major isolectin exhibited an isoelectric point at pH 4.8.This publication has 25 references indexed in Scilit:
- Five structurally related proteins from affinity-purified Maclura pomifera lectinBiochemistry, 1981
- High-performance liquid chromatography of amino acids, peptides and proteins : XXVII. Solvophobic considerations for the separation of unprotected peptides on chemically bonded hydrocarbonaceous stationary phasesJournal of Chromatography A, 1981
- The Semi-Preparative Separation of Peptides on Reversed Phase Silica Packed into Radially Compressed Flexible-Walled ColumnsJournal of Liquid Chromatography, 1981
- The Use of Reversed Phase High Performance Liquid Chromatography for the Structural Mapping of Polypeptides and ProteinsJournal of Liquid Chromatography, 1980
- Purification and properties of the hemagglutinin from Maclura pomifera seedsBiochemistry, 1977
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Derivatization of cross-linked polyacrylamide beads. Controlled introduction of functional groups for the preparation of special-purpose, biochemical adsorbentsBiochemistry, 1969
- Hemagglutinins (Lectins) Extracted from Maclura Pomifera*Vox Sanguinis, 1967
- SIMPLIFIED “DISC” (POLYACRYLAMIDE GEL) ELECTROPHORESIS*Annals of the New York Academy of Sciences, 1964