Radionecrosis of Normal Tissue: Studies on Mouse Tails

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Abstract
Studies on radionecrosis mouse tails demonstrate modifications to the dose necessary for necrosis in 50% of tails (ND50). There was very little reduction in ND50 values for irradiated lengths of tail from 2 cm to almost the whole tail, but there was a sharp increase in dose for lengths less than 1.5 cm. The ND50 was high for unanesthetized mice irradiated in air, due to tissue hypoxia. The hypoxia was reduced by varying amounts by applying heat to the tail, by flowing O2 over the tail surface or anesthetizing the animal. The ingress of O2 through the surface was reduced by placing a clamp round the proximal tail. These features were discussed with reference to the state and possible position of target cells and to the use of this assay technique in comparative studies.