Unfolding Pathways of Individual Bacteriorhodopsins
Top Cited Papers
- 7 April 2000
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 288 (5463) , 143-146
- https://doi.org/10.1126/science.288.5463.143
Abstract
Atomic force microscopy and single-molecule force spectroscopy were combined to image and manipulate purple membrane patches from Halobacterium salinarum. Individual bacteriorhodopsin molecules were first localized and then extracted from the membrane; the remaining vacancies were imaged again. Anchoring forces between 100 and 200 piconewtons for the different helices were found. Upon extraction, the helices were found to unfold. The force spectra revealed the individuality of the unfolding pathways. Helices G and F as well as helices E and D always unfolded pairwise, whereas helices B and C occasionally unfolded one after the other. Experiments with cleaved loops revealed the origin of the individuality: stabilization of helix B by neighboring helices.Keywords
This publication has 48 references indexed in Scilit:
- Structure of bacteriorhodopsin at 1.55 Å resolutionJournal of Molecular Biology, 1999
- CLOSING IN ON BACTERIORHODOPSIN: Progress in Understanding the MoleculeAnnual Review of Biophysics, 1999
- Site-directed spin-labeling reveals the orientation of the amino acid side-chains in the E-F loop of bacteriorhodopsin 1 1Edited by W. BaumeisterJournal of Molecular Biology, 1999
- Electrostatically Balanced Subnanometer Imaging of Biological Specimens by Atomic Force MicroscopeBiophysical Journal, 1999
- Surface structures of native bacteriorhodopsin depend on the molecular packing arrangement in the membrane 1 1Edited by W. BaumeisterJournal of Molecular Biology, 1999
- Electronic properties, stability, and length scales of clustersJournal of Physics: Condensed Matter, 1999
- Structure and function of proteins in G-protein-coupled signal transferBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1996
- Electron-crystallographic Refinement of the Structure of BacteriorhodopsinJournal of Molecular Biology, 1996
- Adhesion Forces Between Individual Ligand-Receptor PairsScience, 1994
- Proton translocation mechanism and energetics in the light-driven pump bacteriorhodopsinBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1993