Adenine specific DNA chemical sequencing reaction
Open Access
- 12 October 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (19) , 7823-7830
- https://doi.org/10.1093/nar/15.19.7823
Abstract
Reaction of DNA with K2PdCI4 at pH2.0 followed by a piperidine workup produces specific cleavage at adenine (A) residues. Product analysis revealed the K2PdCI4 reaction involves selective depurination at adenine, affording an excision reaction analogous to the other chemical DNA sequencing reactions. Adenine residues methylated at the exocyclic amine (N6) react with lower efficiency than unmethylated adenine in an identical sequence. This simple protocol specific for A may be a useful addition to current chemical sequencing reactions.Keywords
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