Identification of Several Clinical Isolates of Dermatophytes Based on the Nucleotide Sequence of Internal Transcribed Spacer 1 (ITS 1) in Nuclear Ribosomal DNA
- 1 May 1999
- journal article
- research article
- Published by Wiley in The Journal of Dermatology
- Vol. 26 (5) , 276-281
- https://doi.org/10.1111/j.1346-8138.1999.tb03472.x
Abstract
Nucleotide sequences of internal transcribed spacer 1 (ITS 1) in nuclear ribosomal DNA from seven morphlogically unidentified dermatophyte isolates were determined. The sequences were compared with those of typical isolates of Trichophyton (T.) mentagrophytes var. interdigitale, T. rubrum, and Epidermophyton floccosum. Two of the isolates were classified as T. rubrum and the other five as T. mentagrophytes var. interdigitale. The results did not conflict with identifications using other molecular techniques, including random amplification of polymorphic DNA (RAPD) analysis and restriction enzyme analysis of mitochondrial DNAs. Thus, the nuclotide sequence of ITS 1 is possibly a good molecular marker for identification of these major anthropophilic dermatophyte species.Keywords
Funding Information
- Japanese Ministry of Education, Science and Culture, C-2 (80190958)
- Kanazawa Medical University (C98-7)
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