Synthesis of vitamin B1 by intestinal bacteria of the rat

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Abstract
Mixed cultures from the rat caecum were grown at 37[degree] in flasks of broth for 1/2-9 days. After each interval some flasks were removed and centrifuged. The bacterial ppt. was dried in vacuo over P2O5 at roomtemp.and the supernatant fluid passed through Seitz filters. Vit. B was assayed separately in each specimen of dry bacteria and of supernatant fluid by the bradycardia method. About 16 I.U. per gm. were synthesized by the bacteria in the young cultures, the amt. gradually diminishing after 3 days'' incubation. No vit. diffused out into the culture medium.