DIFFERENTIAL-EFFECTS OF ELLIPTICINE AND AZA-ANALOGUE DERIVATIVES ON CELL-CYCLE PROGRESSION AND SURVIVAL OF BALB/C 3T3 CELLS RELEASED FROM SERUM STARVATION OR THYMIDINE DOUBLE BLOCK

  • 1 January 1985
    • journal article
    • research article
    • Vol. 45  (8) , 3906-3911
Abstract
10-[Diethylaminopropylamino]-6-methyl-5H-pyrido[3'',4'':4,5]pyrrolo[2,3-g]isoquinoline (BD-40) (NSC-327471D) is an aza-ellipticine derivative with a promising antitumor activity (Marty, et al. 1981) and less toxicity than ellipticine. The effects were compared of ellipticine, several of its analogs, and 2 aza-analogue ellipticine derivatives (BD-40 and BR-1376 [6,11-dimethyl-5H-pyrido[3''-4'':4,5]pyrrolo[2,3-g]isoquinonoline] on cell cycle progression of BALB/c 3T3 mouse cells under different growth conditions. Both durg series stopped cell growth and block cells in G2 phase in exponentially growing cultures and cultures released from a thymidine double block. Long-term viability of these cells was completely suppressed after a short exposure to the drugs. In contrast, while ellipticine and its derivatives caused identical effects in cells recoverying from serum starvation, BD-40 and BR-1376 did not block cells in G2 phase and did not prevent the completion of the first division round occurring after serum addition to quiescent cells. This transient refractory state was accompanied by a total conservation of long-term viability of these cells at least for the next 6 h following serum and drug addition. This lack of effect was not related to an impaired drug uptake by cells recovering from serum starvation or by a dramatic change in drug distribution inside the cells. The nitrogen substitution in the ellipticine heterocycle is an important if not unique feature for the particular effect of the aza-analogs of ellipticine. In contrast to ellipticine derivatives, these compounds require an activation step before exhibiting cytotoxicity.