Effect of Low Levels of Fluoride in Solution on Enamel Demineralization in vitro

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Abstract
The effect of low levels of fluoride in solution on in vitro enamel demineralization has been studied. Extracted human teeth were exposed to 0.1 M lactate solutions (at pH 4.3) which were partially saturated with respect to enamel mineral and which contained between 0.004 and 1 ppm fluoride. Enamel demineralization was monitored using SEM and polarized light microscopy. It was observed that, in the absence of fluoride, rapid enamel demineralization occurred, resulting in the formation of cavitations within 72 hours. Using the same demineralizing medium containing as little as 0.024 and 0.054 ppm fluoride, we observed a remarkable protection of the enamel surface. Subsurface enamel demineralization was, however, observed under these conditions as well as in a solution containing 0.154 ppm fluoride. When the demineralizing solution containing 1 ppm fluoride was used, no mineral loss was detected. The observed inhibition of enamel demineralization was also shown to be associated with a significant uptake of fluoride by the enamel mineral. These observations have been noted to correlate with an increase in solution supersaturation with respect to fluoridated apatitic species. The results obtained were found to be consistent with a proposed hypothesis that the net rate of enamel demineralization will be reduced in a demineralizing medium supersaturated with respect to less soluble fluoridated phases, due to the enhancement of the precipitation rate offluoridated apatitic phases relative to the rate of dissolution of the original enamel surface.