A γδ T cell specific surface receptor (WC1) signaling G0/G1 cell cycle arrest

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Abstract
Three monoclonal antibodies (mAb; SC‐6, SC‐12, and SC‐29) reactive with the γδ T cell‐restricted antigen WC1 were obtained immunizing mice with an ovine interleukin (IL)‐2‐dependent γδ T cell line. These mAb strongly inhibited DNA synthesis in IL‐2‐dependent γδ T cell lines with cell cycle arrest in G0/G1 phase, but did not induce apoptosis. The mAb‐induced growth arrest was reversible, either by removing the mAb or by co‐culture with mitogen or anti‐CD3 in the presence of IL‐2. In contrast, addition of phorbol ester, ionomycin and IL‐2 had no effect on the mAb‐induced growth arrest. The observations define a biologically important role for the cell surface molecule WC1 in the regulation of γδ T cell proliferation and also provide a suitable system to study the relevant signal transduction events.