Quantitation of P-Aminohippuric Acid and N-Acetyl-P-Aminohippuric Acid from Blood by HPLC

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Abstract
P-Aminohippuric acid, N-acetyl-p-aminohippuric acid and p-aminobenzoic acid can be separated by a reverse-phase, isocratic high-pressure liquid chromatographic procedure in < 4 min. The eluent is 10 mM sodium phosphate buffer, pH 3.5, containing 30% methanol. Detection is by absorbance at 270 nm; quantitation is accomplished by peak height. Linear response was obtained from 3-1600 nmols of each compound in aqueous standards and in [human] blood deproteinized with perchloric acid.