Fine specificity, idiotypy, and nature of cloned heavy-chain variable region genes of murine monoclonal rheumatoid factor antibodies.
- 1 November 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (21) , 8293-8297
- https://doi.org/10.1073/pnas.83.21.8293
Abstract
We investigated the immunochemical and molecular characteristics of murine monoclonal rheumatoid factors. Study of the fine specificity of 20 monoclonal rheumatoid factor antibodies shows a wide degree of heterogeneity. However, many express an interstrain cross-reactive idiotype. We show that our rheumatoid factors utilize a restricted set of the heavy-chain variable region (VH) repertoire representing the more 3'' VH families. Preferential expression of 3'' VH families is known to occur early in development. We report the nucleotide sequence of two cloned rheumatoid factor VH genes, Y19-10 (VH J558) and 129-48 (VH 7183) in which no major differences are observed between VH genes encoding the heavy chain of autoantibodies and antibodies against foreign antigens.This publication has 20 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Immunochemical and molecular characterization of regulatory idiotopes expressed by monoclonal antibodies exhibiting or lacking beta 2-6 fructosan binding activity.The Journal of Experimental Medicine, 1985
- Genetic elements used for a murine lupus anti-DNA autoantibody are closely related to those for antibodies to exogenous antigens.The Journal of Experimental Medicine, 1985
- Characterization of an epibody. An antiidiotype that reacts with both the idiotype of rheumatoid factors (RF) and the antigen recognized by RF.The Journal of Experimental Medicine, 1985
- Preferential utilization of the most JH-proximal VH gene segments in pre-B-cell linesNature, 1984
- Delineation of a cross-reactive idiotype on human autoantibodies with antibody against a synthetic peptide.The Journal of Experimental Medicine, 1984
- Site-specific recombination between immunoglobulin D and JH segments that were introduced into the genome of a murine pre-B cell lineCell, 1984
- The immunoglobulin heavy chain variable region (Igh‐V) locus in the mouse. I. One hundred Igh‐V genes comprise seven families of homologous genesEuropean Journal of Immunology, 1984
- Structural Correlates of Idiotypy in the Arsonate SystemAnnals of the New York Academy of Sciences, 1983
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975