Chromosomal localization of structural genes and regulators in wheat by 2D electrophoresis of ditelosomic lines
- 1 November 1985
- journal article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 71 (1) , 31-38
- https://doi.org/10.1007/bf00278250
Abstract
Among the 782 spots observed in two-dimensional gel electrophoresis of denatured proteins from etiolated wheat shoots, 185 were found to be variable between the euploid and 26 ditelosomic lines of ‘Chinese Spring’. Thirty-five structural genes were located on 17 chromosome arms. Numerous intensity changes showing alterations in protein levels were observed and led to the following statements: 1) regulators are frequently found and can be assigned for a same polypeptide to various chromosome arms; 2) for most polypeptides homoeologous arms do not manifest similar effects; 3) nevertheless, when affecting the same polypeptide, homoeologous arms display in most cases identical regulatory effects; 4) gene dosage compensation is observed in only one out of four homoeoallelic situations.Keywords
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