Equilibration of leucine between the plasma compartment and leucyl-tRNA in the heart, and turnover of cardiac myosin heavy chain
- 15 January 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 194 (1) , 365-368
- https://doi.org/10.1042/bj1940365
Abstract
By 30min continuous infusion of [3H]leucine into rats, the specific radioactivities of plasma leucine and tissue-free and tRNA-bound leucine in heart were equal. The specific radioactivity of leucyl-tRNA in heart therefore follows a time course identical with that of plasma leucine soon after the start of infusion. The half-life of cardiac myosin heavy chain (5.5 days) was the same as that reported by other investigators who used the pulse-labelling protocol.This publication has 18 references indexed in Scilit:
- Influence of extraction procedure on size of free amino acid pool of heart muscleAnalytical Biochemistry, 1980
- Early changes in myocardial protein synthesis in vivo in response to right ventricular pressure overload in the dogJournal of Molecular and Cellular Cardiology, 1979
- An ultramicro method of amino acid analyses: Application to studies of protein metabolism in cultured cellsAnalytical Biochemistry, 1979
- [24] Assessment of protein synthesis by the use of aminoacyl-tRNA as precursorPublished by Elsevier ,1979
- Turnover of muscle protein in the fowl (Gallus domesticus). Rates of protein synthesis in fast and slow skeletal, cardiac and smooth muscle of the adult fowlBiochemical Journal, 1978
- Synthesis of guinea-pig cardiac myosin as measured by constant infusionBiochemical Journal, 1978
- Use of the constant infusion technique for measuring rates of protein synthesis in the New Zealand White rabbitBritish Journal of Nutrition, 1977
- Microanalysis of Amino Acids and Their Determination in Biological Material Using Dansyl ChlorideHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1972
- Regulatory significance of transfer RNA charging levels I. Measurements of charging levels in livers of chow-fed rats, fasting rats, and rats fed balanced or imbalanced mixtures of amino acidsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Biochemical Correlates of Cardiac HypertrophyCirculation Research, 1968