A MANOMETRIC ASSAY OF INSULIN AND SOME RESULTS OF THE APPLICATION OF THE METHOD TO SERA AND ISLETCONTAINING TISSUES1

Publisher Website
Google Scholar
Abstract
The use of the manometric measurement of the net gas exchange of rat adipose tissue to quantitate small amounts of insulin is described. The method is simple, does not require that insulin standards be run concomitantly and is capable of measuring quantities of insulin of the order of 0.01 μg. In addition, the method permits the response of the tissue to be observed throughout the time course of the procedure. The importance of this in the measurement of the insulin-like activity of certain biological materials is illustrated. Examples of the application of the technique to the measurement of the insulin-like activity of toad fish islet tissue, human insulinomas, and human sera are given. The interpretation of the results in the case of sera is complicated by the unusual behavior in activity that it exhibits upon dilution and upon storage in the frozen state.