Rapid microorganism identification with on‐slide proteolytic digestion followed by matrix‐assisted laser desorption/ionization tandem mass spectrometry and database searching
- 17 September 2002
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 16 (20) , 1953-1956
- https://doi.org/10.1002/rcm.793
Abstract
A simple method for microorganism identification is proposed in this paper. In this method, no isolation or fractionation of microorganisms and no special search algorithm or database are needed. Experimentally, a sample of the unfractionated intact microorganism is subjected to brief on-slide proteolytic digestion and the digestion product ions detected in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) are selected for tandem mass spectrometry (MS/MS). Database searching using the MS/MS spectrum identifies the microorganism protein, and thereby, according to the source of the protein, the microorganism. The method was demonstrated to be feasible with enterobacteriophage MS2 as the model microorganism. The novel method is simple, rapid, and requires a small quantity of sample. Copyright © 2002 John Wiley & Sons, Ltd.Keywords
This publication has 17 references indexed in Scilit:
- Mass Spectrometry-Based Proteolytic Mapping for Rapid Virus IdentificationAnalytical Chemistry, 2002
- Detection of specific Bacillus anthracis spore biomarkers by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometryRapid Communications in Mass Spectrometry, 2001
- Bioinformatics and Mass Spectrometry for Microorganism Identification: Proteome-Wide Post-Translational Modifications and Database Search Algorithms for Characterization of Intact H. pyloriAnalytical Chemistry, 2001
- Detection of bacteria from biological mixtures using immunomagnetic separation combined with matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometryRapid Communications in Mass Spectrometry, 2001
- Characterization of intact microorganisms by MALDI mass spectrometryMass Spectrometry Reviews, 2001
- MALDI‐TOF mass spectrometry of bacteria*Mass Spectrometry Reviews, 2001
- MALDI Quadrupole Time-of-Flight Mass Spectrometry: A Powerful Tool for Proteomic ResearchAnalytical Chemistry, 2000
- A Practical Ion Trap Mass Spectrometer for the Analysis of Peptides by Matrix-Assisted Laser Desorption/IonizationAnalytical Chemistry, 1996
- Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase geneNature, 1976
- Identification of bacteria using mass spectrometryAnalytical Chemistry, 1975