Evaluation of the Repliscan II System for identification of Enterobacteriaceae

Abstract
Recent improvements prompted the evaluation of Repliscan II by using representative isolates of the population sample previously used to test the system. Isolates (692) representing 8 genera were identified in parallel by the Repliscan II and API20E systems. Isolates given different identifications by the 2 systems were assigned reference identifications by using classical microbiological methods. Repliscan II identified 95.2% correctly, 4.0% incorrectly and 0.7% as unknown. API 20E identified 99.4% correctly, 0.6% incorrectly and none as unknown. Repliscan II correctly identified Salmonella and Shigella spp. to the genus level and isolates of 6 other genera to the species level as follows: Salmonella spp., 100%; Shigella spp., 97.7%; Escherichia spp., 95.2%; Citrobacter spp., 82.1%; Enterobacter spp., 85.2%; Klebsiella spp., 98.6%; Proteus spp., 97.2%; and Serratia spp., 97.9%. These findings indicate that Repliscan II is a significantly improved system and provides acceptable identification of Enterobacteriaceae.

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