Determination of Tenoxicam in Human Plasma using Solid-Phase Extraction and High-Performance Liquid Chromatography with Ultraviolet Detection

Abstract
A sensitive, specific and rapid liquid chromatographic procedure to selectively monitor tenoxicam in human plasma was developed and validated. Plasma samples were acidified and extracted using solid-phase exctration column. The procedure was linear from 0.1 to 10 μ.g/ml with a detection limit of 0.05μg/ml. The coefficient of variation for the procedure is 6.2% and 2.0% for the range of concentrations examinated. This method is suitable for pharmacological, toxicological and pharmacokinetic studies of tenoxicam.