The Precursor of Rat Liver Mitochondrial Glutamate Dehydrogenase has Enzymatic Activity
- 1 July 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 133 (3) , 641-644
- https://doi.org/10.1111/j.1432-1033.1983.tb07511.x
Abstract
The cytosolic precursor for the mitochondrial glutamate dehydrogenase of rat liver was synthesized in a cell-free reticulocyte lysate using mRNA from rat liver. To check whether this precursor had enzymatic activity, a highly sensitive fluorimetric method, which can measure picogram quantities of enzyme, was used together with competitive dissociation of the precursor from an immunoprecipitate with inactive glutamate dehydrogenase. Glutamate dehydrogenase activity, corresponding to that estimated from incorporation of [35S]-methionine, was detected in the precursor. The significance of this finding is discussed.This publication has 16 references indexed in Scilit:
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