Secretory response of dispersed rat submandibular cells. II. Mucin secretion
- 1 March 1980
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 238 (3) , C99-C106
- https://doi.org/10.1152/ajpcell.1980.238.3.c99
Abstract
The secretory response of dispersed rat submandibular cells as it relates to the secretion of D-[1-14C]glucosamine hydrochloride-labeled mucin following sympathomimetic and parasympathomimetic stimulation was evaluated. The adrenergic agonists (-)-norepinephrine and (-)-epinephrine were found to have equal efficacy and potency with a median effective concentration (EC50) of 7.1 x 10(-7) M. (-)-Isoproterenol was found to be acting as a "partial" agonist and had an EC50 of 3.9 x 10(-7) M. (-)-Phenylephrine addition resulted in a small, but significant, secretion of mucin at higher doses tested (10(-4) M--10(-3) M). Neither cholinergic nor alpha-adrenergic receptor stimulation was able to elicit a net increase in the secretion of mucin. However alpha-adrenergic receptor activation in conjunction with beta-adrenergic receptor activation facilitated the rate of secretion. Extracellular Ca2+ and Mg2+ were not required for the secretion of mucin, but extracellular Ca2+ enhanced the rate of secretion following alpha- and beta-adrenergic receptor activation. However extracellular Ca2+ did not enhance mucin secretion following beta-adrenergic receptor activation. Both cellular Ca2+ and beta-adrenergic receptor activation were required to elicit a secretory response following sympathomimetic stimulation.This publication has 10 references indexed in Scilit:
- Secretory response of dispersed rat submandibular cells. I. Potassium releaseAmerican Journal of Physiology-Cell Physiology, 1980
- Functional characteristics of dispersed rat submandibular cells.Proceedings of the National Academy of Sciences, 1979
- Calcium mediation of cholinergic-stimulated amylase release from mouse parotid glandAmerican Journal of Physiology-Cell Physiology, 1979
- IS CALCIUM FINAL MEDIATOR OF EXOCYTOSIS IN RAT PAROTID-GLAND1977
- alpha‐Adrenergic, beta‐adrenergic and cholinergic mechanisms for amylase secretion by rat parotid gland in vitro.The Journal of Physiology, 1976
- The murine sublingual and submandibular mucins their isolation and characterizationBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- Effect of EGTA on Protein Release and Cyclic AMP Accumulation in Rat Parotid GlandCanadian Journal of Physiology and Pharmacology, 1973
- Structure and carbohydrate histochemistry of mammalian salivary glandsJournal of Anatomy, 1962
- Measurement of Cell Growth in Tissue Culture with a Phenol Reagent (Folin-Ciocalteau)Experimental Biology and Medicine, 1956