Biology and Plasticity of CD133+ Hematopoietic Stem Cells
- 1 May 2003
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 996 (1) , 141-151
- https://doi.org/10.1111/j.1749-6632.2003.tb03242.x
Abstract
AC133 (CD133) is a highly conserved antigen expressed on hematopoietic stem cells with unknown function. In order to further characterize CD133+ progenitor cells, we purified CD133+ stem cells using the method of magnetic activated cell sorting (MACS) from healthy adult volunteers mobilized with granulocyte colony‐stimulating growth factor (G‐CSF) to a mean purity of 94%. The purified CD133+ cells highly engrafted NOD/SCID mice. In addition, unseparated mononuclear cells or CD133+ stem cells isolated from the bone marrow of transplanted NOD/SCID mice gave rise to engraftment of secondary recipients. Upon ex vivo culture of purified CD133+ cells with FLT3/Flk2 ligand (FL) and interleukin‐6 (IL‐6), a plastic‐adherent cell population could be observed after 6 weeks in culture. These adherent cells did not express CD34 or CD133 antigens on their surface, nor did they express markers for endothelial, mesenchymal, or dendritic cells. After incubation of these adherent cells with stem cell factor (SCF), non‐adherent cells were observed which partially co‐expressed CD133, but were negative for CD34. These nonadherent CD34− cells showed a high engraftment capacity in NOD/SCID mice. From our results, we conclude that CD133 might be a marker of early progenitors with a high NOD/SCID engraftment potential. The fact that CD133+ hematopoietic progenitors can give rise to an adherent population which is CD133− and CD34− and that these cells can again give rise to a CD133+CD34− stem cell population with high NOD/SCID engraftment potential indicates the plasticity of hematopoietic precursors. CD133+ stem cells might be useful for research and for clinical application.Keywords
This publication has 15 references indexed in Scilit:
- Identification of a novel class of human adherent CD34− stem cells that give rise to SCID-repopulating cellsBlood, 2003
- AC133 expression in human stem cellsLeukemia, 2001
- The Monoclonal Antibody W7C5 Defines a Novel Surface Antigen on Hematopoietic Stem CellsAnnals of the New York Academy of Sciences, 2001
- Signal-regulatory protein α (SIRPα) but not SIRPβ is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34+CD38−hematopoietic cellsBlood, 2001
- Megadose transplantation of purified peripheral blood CD34+progenitor cells from HLA-mismatched parental donors in childrenBone Marrow Transplantation, 2001
- Transplantation of highly purified HLA-identical sibling donor peripheral blood CD34+ cells without prophylactic post-transplant immunosuppression in adult patients with first chronic phase chronic myeloid leukemia: results of a phase II studyBone Marrow Transplantation, 2000
- Expression of VEGFR-2 and AC133 by circulating human CD34+ cells identifies a population of functional endothelial precursorsBlood, 2000
- A frameshift mutation in prominin (mouse)-like 1 causes human retinal degenerationHuman Molecular Genetics, 2000
- Primers and protocols for standardized detection of minimal residual disease in acute lymphoblastic leukemia using immunoglobulin and T cell receptor gene rearrangements and TAL1 deletions as PCR targets Report of the BIOMED-1 CONCERTED ACTION: Investigation of minimal residual disease in acute leukemiaLeukemia, 1999
- A Response to AC133 Hematopoietic Stem Cell Antigen: Human Homologue of Mouse Kidney Prominin or Distinct Member of a Novel Protein Family?Blood, 1998