Mapping of the full profile of T cell allorecognition regions on HLA‐DR2β subunit

Publisher Website
Google Scholar
Abstract
Alloreactive human T cell lines were developed by repeated in vitro restimulation with alternate allogeneic cells of similar DR2 serotype, but differing at other HLA loci. These polyclonal T cells recognized DR specificities in common with all DR2 serotypes. The allorecognition profiles of DR2β by the T cell lines were mapped by consecutive uniform‐sized overlapping peptides encompassing the entire extracellular and intracellular regions of the DRP subunit. It was found that the T cell allorecognition sites are focused around the first and third polymorphic regions of the N‐terminal domain, and interestingly, include conserved areas in the DRβ second domain as well as the intracellular segment β222–237. These findings have important implications for allorecognition. Comparison with the previous analysis of antibody‐binding regions of DR2β revealed some similarities and differences in the antibody and T cell alloresponses to this histocompatibility antigen.