Visualization of barley β‐glucan degrading isozymes after gel isoelectric focusing

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Abstract
A simple method to study the polymorphism of barley (Hordeum vulgare L.) β‐(1–3, 1–4)‐glucanases (specific enzymes of barley β‐glucan hydrolysis) is described. Proteins of a crude extract of germinated barley kernels were separated in an immobilized pH gradient in two pH ranges (pH 3–10.5 and pH 4–7). β‐glucanases were visualized by contact printing with a polyacrylamide gel containing β‐glucan or lichenan. Patterns of β‐glucanases were revealed by staining with Congo Red with resultant clear zones on a stained background. Various conditions of germination, extraction and visualization were investigated. New isozyme bands could be detected and their nature and origin discussed.

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