Epigenetic status of human embryonic stem cells
- 1 May 2005
- journal article
- research article
- Published by Springer Nature in Nature Genetics
- Vol. 37 (6) , 585-587
- https://doi.org/10.1038/ng1556
Abstract
We examined the allele-specific expression of six imprinted genes and the methylation profiles of three imprinting control regions to assess the epigenetic status of human embryonic stem cells. We identified generally monoallelic gene expression and normal methylation patterns. During prolonged passage, one cell line became biallelic with respect to H19, but without loss of the gametic methylation imprint. These data argue for a substantial degree of epigenetic stability in human embryonic stem cells.Keywords
This publication has 15 references indexed in Scilit:
- Selective loss of imprinting in the placenta following preimplantation development in cultureDevelopment, 2004
- Epigenetic risks related to assisted reproductive technologies: Epigenetics, imprinting, ART and icebergs?Human Reproduction, 2003
- Preimplantation Human Embryos and Embryonic Stem Cells Show Comparable Expression of Stage‐Specific Embryonic AntigensThe International Journal of Cell Cloning, 2002
- Epigenetic Instability in ES Cells and Cloned MiceScience, 2001
- Culture of Preimplantation Mouse Embryos Affects Fetal Development and the Expression of Imprinted Genes1Biology of Reproduction, 2001
- Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo cultureNature Genetics, 2001
- Differential Effects of Culture on Imprinted H19 Expression in the Preimplantation Mouse Embryo1Biology of Reproduction, 2000
- A maternally methylated CpG island in KvLQT1 is associated with an antisense paternal transcript and loss of imprinting in Beckwith–Wiedemann syndromeProceedings of the National Academy of Sciences, 1999
- Two novel genes in the center of the 11p15 imprinted domain escape genomic imprinting.Human Molecular Genetics, 1999
- Functional imprinting and epigenetic modification of the human SNRPN geneHuman Molecular Genetics, 1993