Genetic studies on the β subunit of Escherichia coli RNA polymerase
- 1 June 1986
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 203 (3) , 492-495
- https://doi.org/10.1007/bf00422075
Abstract
Summary The assembly of RNA polymerase was studied in Escherichia coli mutants encoding large N-terminal amber fragments of the β subunit. Whereas the removal of up to 20% of the carboxy-terminus does not prevent the formation of premature core enzyme, the amber fragments seem to interfere with holoenzyme production. These studies permit, therefore, the localization of a region on the β polypeptide involved in sigma binding.Keywords
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