Genetic studies on the β subunit of Escherichia coli RNA polymerase
- 1 June 1986
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 203 (3) , 487-491
- https://doi.org/10.1007/bf00422074
Abstract
We have previously isolated an E. coli derivative carrying a small internal deletion (Δ(rpoB) 1570-1) of the β structural gene. This RNA polymerase deletion mutant has no noticeable phenotype other than a slightly increased generation time in minimal medium. The deletion, which removes about 165 bp, has been localised to between codons 965 and 1,083, indicating it excises part of a tandem repeat structure present in the C-terminal region of β. Analysis in vitro of purified RNA polymerase from the deletion mutant indicates that this enzyme has an altered promoter selectivity. These observations allow locatisation of a site on the β polypeptide of E. coli RNA polymerase involved in transcriptional initiation.This publication has 30 references indexed in Scilit:
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