Effects of Anaerobiosis and Nitrate on the Expression of Succinate Dehydrogenase and Enzymes Associated with Nitrogen Metabolism in Klebsiella pneumoniae

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Abstract
The low histidase activity found in anaerobic, N-limited cultures of K. pneumoniae is due to repression of the right-hand hut operon. The effects of NO3- on the aerobic and anaerobic expression of catabolite- and NH4+ repressible enzymes in this organism was also examined. NO3- permitted anaerobic growth of K. pneumoniae in minimal containing histidine as the sole C source, and histidase and succinate dehydrogenase were derepressed during anaerobic growth in histidine/NO3- medium. Use of sucrose rather than histidine as the C source reversed the effects of NO3- and repressed histidase and succinate dehydrogenase activities. Anaerobic growth in sucrose/NO3- medium also uncoupled the expression of urease and glutamine synthetase.