Construction of expression plasmids for the fusion protein of Sendai virus, and their expression inE. colicells and eucaryotic cells
- 12 March 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 262 (1) , 39-41
- https://doi.org/10.1016/0014-5793(90)80148-c
Abstract
To examine the properties and the role of the fusion protein (F) of Sendai virus at the molecular level, a plasmid, pUC-F, was constructed by inserting cDNA for the F protein into a pUC vector. Upon induction of E. coli cells transformed with pUC-F, a new protein was obtained, which was identified as Fo on Western blot analysis. The cDNA fragment for the F gene was excised from pUC-F and inserted into an eucaryotic expression vector, pSVL, to yield pSVL-F. COS-1 cells transfected with pSVL-F gave a band on SDS-gel electrophoresis which corresponded to the size of the Fo proteinKeywords
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