STUDIES ON THE KINETICS OF PROTEIN SYNTHESIS IN YEAST

Publisher Website
Google Scholar
Abstract
The kinetics of incorporation of S35O4[long dash] and C14 amino acids have been followed in exponentially growing cultures of yeast. Within a few seconds, the isotopes are incorporated into protein, appearing first in the ribosomal fraction as nascent protein. After 80 seconds'' exposure of growing yeast cells to C14-labeled amino acids or S35O4[long dash], the addition of unlabeled amino acids or unlabeled cysteine, methionine, and SO4[long dash] caused a rapid decrease in the concentration of C14 or S35, respectively, held by the ribosomes. This response indicated that nascent protein synthesized on the ribosomes is displaced quickly upon completion of its synthesis into the soluble protein pool. Nascent protein represents only a small fraction of the ribosomal protein; however, its turnover rate is sufficient to account for the over-all rate of protein synthesis of the cell. The methods employed furnish an estimate of the kinetics of synthesis of the structural protein of ribosomes.

This publication has 8 references indexed in Scilit: