Synthesis of the Brain‐Specific S‐100 Protein in a Cell‐Free System from Wheat Embryo Programmed with Poly(A)‐Containing RNA from Rabbit Brain

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Abstract
Polyadenylated polysomal RNA was prepared from rabbit cerebral hemispheres using phenol extraction and chromatography on oligo(dT)-cellulose. This RNA directed the synthesis of the brain-specific S-100 protein in cell-free extracts from wheat embryo. S-100 protein was absent from the products of endogenous incorporation and from a reaction programmed with kidney mRNA. These results suggest that S-100 protein mRNA contains a poly(A) sequence and rule out the necessity of a brain-specific factor for translation of S-100 protein mRNA.