Methods for the Preparation of Purified Granulopoiesis-Inhibiting Factor (Chalone)

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Abstract
Methods which may be useful for the preparation of chalone-like granulopoietic inhibitor(s) are described. Details are given for preparation of crude starting material from various sources, primary extraction and crude fractionation techniques, gel chromatography, anion exchange chromatography and thiol-binding chromatography. Possibilities for the chromatographic use of marker substances are discussed and methods for obtaining the factor in radioactively labeled form are given. Some of the stability problems encountered during working and storage of granulopoiesis-inhibiting factor (GIF) are discussed. Simple chemical techniques for the purification of GIF for investigators with mainly biological interests are provided.