Purification and characterization of ubiquitin from mammalian testis
- 24 April 1984
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 169 (2) , 199-204
- https://doi.org/10.1016/0014-5793(84)80318-x
Abstract
Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH2-terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved methods for radioimmunoassay of ubiquitin in tissue extracts are also discussed.Keywords
This publication has 23 references indexed in Scilit:
- An electrophoretic investigation of mammalian spermatid-specific nuclear proteinsReproduction, 1982
- Free ubiquitin is a non-histone protein of trout testis chromatinNature, 1978
- The isolation and identification of ubiquitin from the high mobility group (HMG) non‐histone protein fractionFEBS Letters, 1978
- Amino-terminal sequence identity of ubiquitin and the nonhistone component of nuclear protein A24Biochemical and Biophysical Research Communications, 1977
- Molecular conservation of 74 amino acid sequence of ubiquitin between cattle and manNature, 1975
- Complete amino acid sequence of ubiquitin, an adenylate cyclase stimulating polypeptide probably universal in living cellsBiochemistry, 1975
- Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells.Proceedings of the National Academy of Sciences, 1975
- Comparison of Nucleolar Proteins of Normal Rat Liver and Novikoff Hepatoma Ascites Cells by Two-Dimensional Polyacrylamide Gel ElectrophoresisProceedings of the National Academy of Sciences, 1973
- Modification of Histones during Spermiogenesis in Trout: A Molecular Mechanism for Altering Histone Binding to DNAProceedings of the National Academy of Sciences, 1970
- High resolution acrylamide gel electrophoresis of histonesArchives of Biochemistry and Biophysics, 1969