GAP‐43 mRNA in Mouse Motoneurons Undergoing Axonal Sprouting in Response to Muscle Paralysis or Partial Denervation
- 1 June 1996
- journal article
- Published by Wiley in European Journal of Neuroscience
- Vol. 8 (6) , 1240-1248
- https://doi.org/10.1111/j.1460-9568.1996.tb01292.x
Abstract
To test the hypothesis that collateral sprouting of motoneurons can occur without the intervention of metabolic changes in the cell body, we examined the levels of growth-associated protein 43 (GAP-43) mRNA in mouse motoneurons induced to sprout by muscle inactivity (following marcaine or botulinum toxin treatment) or by partial denervation. GAP-43 mRNA was selected as an appropriate marker for cell body metabolic changes because it is expressed at low levels in mature motoneurons, but is strongly expressed during developmental or regenerative axonal growth in motoneurons. Sprouting motoneurons were identified by retrograde labelling with fluorescent tracers applied to the muscle in which sprouting occurred. Both a full-length cDNA probe and an oligonucleotide probe were used for in situ hybridization. We were unable to detect any significant increases in GAP-43 mRNA levels in fluorescent motoneurons after any treatment, except 4 days after partial denervation (but not at 2 or 8 days). This amounted to a 1.6-fold increase in signal level compared to control motoneurons, while presumed axotomized motoneurons in the same spinal cords displayed on average an 8. 7-fold increase. We conclude that collateral sprouting can occur in motoneurons without a detectable increase in cell body levels of GAP-43 mRNA. The modest increase observed in the 4-day partial denervation situation may be a response to the more vigorous and extensive nodal axonal sprouting occurring in these motoneurons. Our results do not deny a role for pre-existing GAP-43 in collateral sprouting, but support the hypothesis that sprouting can occur in motoneurons without necessarily requiring increase GAP-43 mRNA levels in the cell body.Keywords
This publication has 46 references indexed in Scilit:
- Increase in Neurite Formation and Acetylene line Release by Transfection of Growth-Associated Protein-43 cDNA into NG108-15 CellsJournal of Neurochemistry, 2006
- Absence of persistent spreading, branching, and adhesion in GAP-43-depleted growth cones.The Journal of cell biology, 1995
- GAP-43 and p75NGFR immunoreactivity in presynaptic cells following neuromuscular blockade by botulinum toxin in ratJournal of Neurocytology, 1994
- Expression of NGF receptor and GAP‐43 mRNA in DRG neurons during collateral sprouting and regeneration of dorsal cutaneous nervesJournal of Neurobiology, 1994
- Expression of GAP‐43 mRNA in Mouse Spinal Cord Following Unilateral Peripheral Nerve Damage: is There a Contralateral Effect?European Journal of Neuroscience, 1993
- GAP‐43 mRNA in Rat Spinal Cord and Dorsal Root Ganglia Neurons: Developmental Changes and Re‐expression Following Peripheral Nerve InjuryEuropean Journal of Neuroscience, 1992
- Inhibition of nerve growth factor-induced B-50GAP-43 expression by antisense oligomers interferes with neurite outgrowth of PC12 cellsBiochemical and Biophysical Research Communications, 1992
- B‐50 (GAP‐43): Biochemistry and Functional Neurochemistry of a Neuron‐Specific PhosphoproteinJournal of Neurochemistry, 1991
- Electrophysiological and morphological investigation of bupivacaine-induced myopathy and terminal sprouting in the ratExperimental Neurology, 1982
- Temporal parameters of axon “sprouting” in the brain of the adult ratExperimental Neurology, 1977