MODIFIED FLUORIMETRIC FLOW-INJECTION-ANALYSIS (FIA) METHOD FOR THE DETERMINATION OF (1-3) (1-4)-B-D-GLUCAN
Open Access
- 10 September 1987
- journal article
- research article
- Published by Chartered Institute of Brewers and Distillers in Journal of the Institute of Brewing
- Vol. 93 (5) , 396-398
- https://doi.org/10.1002/j.2050-0416.1987.tb04525.x
Abstract
A simple system has been constructed for the quantitative determination of barley β-glucans. Measurements were made by the flow-injection-analysis (FIA) technique using the fluorescent dye Calcofluor as a specific reagent for (1·3) (1·4)–B—D—linkages. In this paper a single-line system is described involving only one pump samples of β-glucan being directly injected into the reagent stream. The reaction results in an increased intensity of fluorescence detected by a flow-through-cell fluorimeter and evaluated as peak heights or peak areas by a printer-plotter. The analysis is completed within 20s. The operational parameters were set during an optimization procedure.Keywords
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