Studies on heart. XXI. Amino acid sequence of antiarrhythmic peptide (AAP) isolated from atria.

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Abstract
The amino acid sequence of [bovine] atrial peptide, AAP, that improves the rhythmicity of cultured myocardial cell clusters, was established as Gly-Pro-4Hyp-Gly-Ala-Gly. This was accomplished by the sequence analyses with dansyl-Edman degradation and carboxypeptidase Y digestion on the isolated native peptide. The hexapeptide and its several constituent peptides were synthesized by using dicyclohexylcarbodiimide as a condensing agent. Synthetic AAP was chemically and biologically indistinguishable from the native one. In a structure-activity correlation of AAP, the whole molecule of this peptide was evidently essential for its antiarrhythmic activity and its promoting effect on spreading phenomenon of myocardial cells in culture.