Expression of the Middle Component RNA of Cowpea Mosaic Virus in vivo

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Abstract
Upon infection of cowpea mesophyll protoplasts with cowpea mosaic virus (CPMV), the only M RNA-encoded proteins detected so far have been the two capsid proteins VP37 and VP23. We now report the detection of a Mr 60,000 (60K) precursor to both capsid proteins in infected protoplasts cultured in the presence of zinc ions. Furthermore a M RNA-encoded 48K protein was detected in the membrane fraction of infected cells using antisera raised against synthetic peptides. The results obtained indicate that, just like the bottom component RNA, the M RNA of CPMV is translated in vivo into a polyprotein from which proteins are derived by proteolytic cleavage.