Antibodies Against the Genome-Linked Protein VPg of Cowpea Mosaic Virus Recognize a 60,000-Dalton Precursor Polypeptide
- 1 March 1982
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 41 (3) , 1083-1088
- https://doi.org/10.1128/jvi.41.3.1083-1088.1982
Abstract
We have prepared a rabbit antiserum specifically directed against the genome-linked protein (VPg) of cowpea mosaic virus by injecting an hydrolysate of purified virion RNA. Using this antiserum as a probe in combination with “Western” (protein) blots of subcellular fractions of cowpea mosaic virus-infected cowpea ( Vigna unguiculata ) cells, we have detected a bottom component RNA-encoded, 60,000-dalton polypeptide which is membrane bound and presumably represents the immediate precursor of VPg.This publication has 40 references indexed in Scilit:
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Comparison of in vivo and in vitro translation of Cowpea Mosaic Virus RNAsBiochemical and Biophysical Research Communications, 1981
- The genome-linked protein of picornaviruses. VII. Genetic mapping of poliovirus VPg by protein and RNA sequence studiesCell, 1980
- Nucleotide Sequences Adjacent to the Proteins Covalently Linked to the Cowpea Mosaic Virus GenomeEuropean Journal of Biochemistry, 1979
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Evidence for a Protein Covalently Linked to Tobacco Ringspot Virus RNAJournal of General Virology, 1979
- Protein Bound to the Genome RNAs of Cowpea Mosaic VirusEuropean Journal of Biochemistry, 1978
- Presence of a Covalently Linked Protein on Calicivirus RNAJournal of General Virology, 1978
- Protein covalently linked to foot-and-mouth disease virus RNANature, 1977
- The location of the polio genome protein in viral RNAs and its implication for RNA synthesisNature, 1977