Genomic cloning and preliminary characterization of the human thymidine kinase gene.
- 1 November 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (21) , 6528-6532
- https://doi.org/10.1073/pnas.80.21.6528
Abstract
The cloning of the human cytoplasmic thymidine kinase (tk-C, EC 2.7.1.21) gene and its preliminary characterization are described. The tk-C sequences were isolated from a phage genomic library made from DNA of a transfected mouse cell carrying the human tk-C gene. The human transforming sequences were identified by homology with human Alu sequences. Six recombinant phages were isolated and 5 were competent to transfer human TK-C activity to TK-deficient mouse cells when transferred in pairs. Conclusively, sequences homologous to these clones are present in all human TK+ transformants examined. The maximal size of the tk-C gene is estimated to be 14 kilobase pairs and its minimal size to be between 4 and 5 kilobase pairs. The gene contains many noncoding inserts and numerous Alu sequences.This publication has 23 references indexed in Scilit:
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