Molecular characterization of Blastocystis isolates in the Philippines by riboprinting
- 11 May 2005
- journal article
- Published by Springer Nature in Zeitschrift Fur Parasitenkunde-Parasitology Research
- Vol. 96 (4) , 253-257
- https://doi.org/10.1007/s00436-005-1344-4
Abstract
Extensive genomic polymorphism has been demonstrated among morphologically identical Blastocystis isolates. To this end, 32 Blastocystis isolates from the Philippines (12 from humans, 12 from pigs and 8 from chickens) were analyzed genetically by riboprinting or restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR) amplified small subunit rDNA. Three distinct riboprint patterns were observed from the HinfI digestion, while four patterns resulted from the RsaI digestion of Blastocystis SSU rDNA. Restriction fragment profiles between Blastocystis isolates from different hosts were generally different from each other. However, Blastocystis isolates within each host group were practically the same. Cluster analysis of the riboprint patterns revealed seven distinct groups of the Blastocystis isolates, including a zoonotic strain. These results demonstrate the genetic heterogeneity of Blastocystis in the Philippines and a support to the idea of the organism’s zoonotic potential.Keywords
This publication has 23 references indexed in Scilit:
- PCR-based identification of zoonotic isolates of Blastocystis from mammals and birdsMicrobiology, 2004
- Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countriesZeitschrift Fur Parasitenkunde-Parasitology Research, 2004
- MOLECULAR COMPARATIVE STUDIES AMONG BLASTOCYSTIS ISOLATES OBTAINED FROM HUMANS AND ANIMALSJournal of Parasitology, 2003
- Molecular characterization of Blastocystis isolates from primatesVeterinary Parasitology, 2003
- Zoonotic genotypes of Blastocystis hominis detected in cattle and pigs by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA geneZeitschrift Fur Parasitenkunde-Parasitology Research, 2003
- Genomic polymorphism among strains and development of subtype-specific diagnostic primersMolecular and Cellular Probes, 1998
- Five subgroups of Blastocystis hominis isolates from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR‐amplified 16S‐like rDNATropical Medicine & International Health, 1997
- Differentiation of Entamoeba histolytica and E. dispar DNA from cysts present in stool specimens by polymerase chain reaction: its field application in the PhilippinesZeitschrift Fur Parasitenkunde-Parasitology Research, 1996
- DNA Polymorphism Revealed by Arbitrary Primers Polymerase Chain Reaction Among Blastocystis Strains Isolated from Humans, a Chicken, and a ReptileThe Journal of Eukaryotic Microbiology, 1996
- Blastocystis in Humans and Animals: Morphology, Biology, and EpizootiologyAdvances in Parasitology, 1993