Glutamate and kainate increase intracellular sodium activity in leech neuropile glial cells
- 1 January 1989
- Vol. 2 (1) , 51-54
- https://doi.org/10.1002/glia.440020106
Abstract
Na+‐selective, double‐barrelled microelectrodes were used to measure intracellular Na+ activity (a) and membrane potential (Em) in neuropile glial cells of isolated segmental ganglia in the leech Hirudo medicinalis. Bath application of glutamate (10−3 M) resulted in membrane depolarizations of about 5 mV and a concomitant increase of a by between 2 and 10 mM. Kainate (10−4 M) elicited depolarizations of up to 40 mV amplitude followed by a prominent afterhyperpolarization. During kainate, a increased by 7 to 25 mM. In contrast to glutamate, an initial decrease of a was detected during the action of kainate. N‐methyl‐D‐aspartate (NMDA, 10−5–10−3M) had no effect of Em and a. The results indicate that leech glial cells have a kainate‐preferring non‐NMDA glutamate receptor.Keywords
This publication has 13 references indexed in Scilit:
- Kainic acid evokes a potassium efflux from astrocytesNeuroscience, 1988
- Glutamate opens Na+/K+ channels in cultured astrocytesGlia, 1988
- Electrogenic glutamate uptake is a major current carrier in the membrane of axolotl retinal glial cellsNature, 1987
- Glutamate depolarization of glial cells in Necturus optic nerveNeuroscience Letters, 1986
- Aspartate, glutamate and γ-aminobutyric acid depolarize cultured astrocytesNeuroscience Letters, 1984
- Excitatory amino acids directly depolarize rat brain astrocytes in primary cultureNature, 1984
- Different types of potassium transport linked to carbachol and γ-aminobutyric acid actions in rat sympathetic neuronsNeuroscience, 1984
- Depolarization of cultured oligodendrocytes by glutamate and GABANeuroscience Letters, 1984
- Fluorescence marking of neuropile glial cells in the central nervous system of the leech Hirudo medicinalisCell and tissue research, 1980
- STRUCTURE‐ACTIVITY STUDIES ON AN EXCITATORY RECEPTOR FOR GLUTAMATE ON LEECH RETZIUS NEURONESBritish Journal of Pharmacology, 1980