Heterogeneous denaturation of enzymes: A distributed activation energy model with nonuniform activities

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Abstract
A model is proposed for the denaturation of enzymes based on heterogeneous enzyme subpopulations with nonuniform initial activities and nonzero final activities. The more frequently used simple first‐order deactivation model is a special case of the model corresponding to the standard deviation of Gaussian distribution of activation energies approaching zero. Parametric studies made with the model are capable of simulating the reported trends in experimental data on the thermal profile and activity time behavior of native as well as modified enzymes with a minimum number of adjustable parameters. The model predictions are found to be in good agreement with data reported in the literature.