Survival of Escherichia coli in freshwater: β-D-Glucuronidase activity measurements and characterization of cellular states

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Abstract
A rapid enzyme assay measuring β-D-glucuronidase activity of Escherichia coli was tested in survival experiments after discharge of E. coli in river water. Enzyme activity was compared with several analyses performed to characterize cellular states under stressful conditions. Enzyme activity remained stable under starvation and light stress conditions despite losses of culturability, respiratory activity, and cytoplasmic membrane integrity. β-D-Glucuronidase activity followed the pattern of genetic and morphologic cell integrity. The tested enzyme assay seems well adapted to study the fate of fecal coliforms in survival experiments, and appears to be a rapid and efficient way to estimate the microbiological quality of surface waters.Key words: β-D-glucuronidase activity, Escherichia coli, survival, freshwater, cellular states.