SUGAR CHAIN OF ALPHA-FETOPROTEIN PRODUCED IN HUMAN YOLK-SAC TUMOR

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 43  (10) , 4691-4695
Abstract
The carbohydrate moiety of .alpha.-fetoprotein purified from human yolk sac tumors grown in nude mice was quantitatively released from the polypeptide chain as oligosaccharides by hydrazinolysis. The oligosaccharides were separated into a neutral and 2 acidic oligosaccharides by paper electrophoresis. By sequential exoglycosidase digestion in combination with per-O-methylation study and periodate oxidation, their structures were determined to be: Gal.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 2Man.alpha.1 .fwdarw. 6(GlcNAc.beta.1 .fwdarw. 4) (Gal.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 2Man.alpha.1 .fwdarw. 3)Man .beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 4(Fuc.alpha.1 .fwdarw. 6)GlcNAcOT, Gal.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 2Man.alpha.1 .fwdarw. 6(GlcNAc.beta.1 .fwdarw. 4) (Sia.alpha.2 .fwdarw. 6Gal.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 2Man.alpha.1 .fwdarw. 3)Man.beta.1 .fwdarw. 4GLcNAc.beta.1 .fwdarw. 4(Fuc.alpha.1 .fwdarw. 6)GlcNacOT; and Sia.alpha.2 .fwdarw. 6Gal.beta.1 .fwdarw. GlcNAc.beta.1 .fwdarw. 2Man.alpha.1 .fwdarw. 6(GlcNAc.beta.1 .fwdarw. 4) (Sia.alpha.2 .fwdarw. 6Gal.beta.1 .fwdarw. 4GlcNAc.beta.a .fwdarw. 2 Man.alpha.1 .fwdarw. 3)Man.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 4(Fuc.alpha.1 .fwdarw. 6)GlcNAcOT, in which Gal is galactose, GlcNac is N-acetylglucosamine, Man is mannose, Fuc is fucose, Sia is sialic acid, and Subscript OT is the NaB3H4-reduced oligosaccharide.

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