Activation and Inhibition of Ribulose 1,5-Diphosphate Carboxylase by 6-Phosphogluconate
Open Access
- 1 October 1973
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 52 (4) , 373-379
- https://doi.org/10.1104/pp.52.4.373
Abstract
Ribulose 1,5-diphosphate carboxylase, when activated by preincubation with 1 mm bicarbonate and 10 mm MgCl2 in the absence of ribulose 1,5-diphosphate, remains activated for 20 minutes or longer after reaction is initiated by addition of ribulose diphosphate. If as little as 50 μm 6-phosphogluconate is added during this preincubation period, 5 minutes before the start of the reaction, a further 188% activation is observed. However, addition of 6-phosphogluconate at the same time or later than addition of ribulose diphosphate, or at any time with 50 mm bicarbonate, gives inhibition of the enzyme activity. Possible relevance of these effects in vivo regulatory effects is discussed.Keywords
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