Defining human ERAD networks through an integrative mapping strategy
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Open Access
- 27 November 2011
- journal article
- research article
- Published by Springer Nature in Nature Cell Biology
- Vol. 14 (1) , 93-105
- https://doi.org/10.1038/ncb2383
Abstract
Proteins that fail to correctly fold or assemble into oligomeric complexes in the endoplasmic reticulum (ER) are degraded by a ubiquitin- and proteasome-dependent process known as ER-associated degradation (ERAD). Although many individual components of the ERAD system have been identified, how these proteins are organized into a functional network that coordinates recognition, ubiquitylation and dislocation of substrates across the ER membrane is not well understood. We have investigated the functional organization of the mammalian ERAD system using a systems-level strategy that integrates proteomics, functional genomics and the transcriptional response to ER stress. This analysis supports an adaptive organization for the mammalian ERAD machinery and reveals a number of metazoan-specific genes not previously linked to ERAD.Keywords
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