The use of radioactive isotopes in immunological investigations. 2. The fate of injected 32P-containing proteins

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Abstract
P32-containing proteins were used to study the fate of the intraven. injd. antigens lipovitellin and vitellin, which were obtained from eggs laid by hens injd. with p32-containing inorganic phosphate, and artificially phosphorylated human serum proteins. The injd. proteins quickly disappeared from the blood stream. Less than 25% of the injd. phosphorylated human serum proteins was detectable in the blood 3 hrs. after the injn., and the disappearance of vitellin and lipovitellin was even more rapid, since only about 12% of the injd. protein could be detected in the blood 5 min. after the injn. Appreciable amts. of p32 were found in the liver and lungs of 2 rabbits 1 min. after the injn. of a suspension of vitellin. The presence of this material in the lungs was possibly due to the retention by the lung capillaries of vitellin which was precipitated when the material was introduced into the blood stream.