HISTOCHEMICAL STUDY OF A DERMATOPHYTE UNDER AN ELECTRON MICROSCOPE

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Abstract
Histological sections of T. mentagrophytes stained by chromic acid-silver methenamine, periodic acid-silver methenamine, periodic acid-thiocarbohydrazide-silver methenamine, periodic acid-silver methenamine, periodic acid-thiocarbohydrazide-silver proteinate or alkaline Bi showed the cell walls densely impregnated and clearly defined. With alkaline Bi the cell walls revealed fine granular structures throughout, microfibrous structures in the inner half of the cell wall, and coarse granular structures arranged close together on the outer surface of the plasma membrane. Based on experiments involving acetylation, saponification, periodic acid oxidation and chitinase digestion, these structures are composed mainly of polysaccharides containing vic-glycol groups supported by chitin. Electron micrographs of the general structures of the fungal cells were obtained from sections which were treated with alkaline Bi, combined with uranyl acetate, followed by the lead citrate poststaining technique.

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