Phlebia radiata laccase forms induced by veratric acid and xylidine in relation to lignin peroxidase and manganese‐dependent peroxidase

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Abstract
White‐rot basidiomycete Phlebia radiata grown in the nitrogen‐poor liquid medium was tested for the inducing effect of 2,5‐xylidine and veratric acid on laccase production and properties of its preparations. Also lignin peroxidase and manganese‐dependent peroxidase were assayed under the same conditions. The maximum of laccase activity for veratric acid as a stimulator was done much earlier than that of xylidine. It was very sharp and disappeared quickly. At that time only weak lignin peroxidase and manganese‐dependent peroxidase activities were noticed. The maximum of laccase induced by xylidine was observed much later and kept longer. Both laccase preparations showed the same properties. For biotechnological reasons, the production of laccase induced by the nontoxic veratric acid is much more economic and better acceptable than that induced by xylidine.